Dynamic holographic optical tweezers pdf

Introduction the ability to move and sort single cells is highly sought after in the biomedical and biological community. Design strategies for optimizing holographic optical. April 21, 2012 optical trapping is an increasingly important technique for controlling and probing the structure. Holographic optical tweezers 4 multipoint microscale velocimetry 31, it bears repeating that optical forces are, in the end, still relatively weak, and so for some purposes it would be reasonable to combine the use of optical forces to allow sophisticated, integrated assembly of components with the use of other forces for actuation. Hot is one of the most expected techniques for investigations of. Controlled mechanical motions of microparticles in optical tweezers. Request pdf dynamic holographic optical tweezers optical trapping is an increasingly important technique for controlling and probing matter at length scales ranging from nanometers to. Grier james franck institute and institute for biophysical dynamics the university of chicago, chicago, il 60637 dated. We here propose an apparatus based on holographic optical tweezers to put on a target cell a two or threedimensional custombuilt pattern of beads, with respect to the target cell shape, with both temporal and spatial dynamic control of each contact. Holographic optical tweezers, spatial light modulators, optical design.

The optical tweezers are separated from the zeroth order diffraction by x 01 f 1 f 3 f 2. Holographic optical trapping of aerosol droplets d. However, optical tweezers are limited by high optical power requirements. Dynamic cell and microparticle control via optoelectronic. Optical tweezers principles and applications practice and applications of optical tweezers. Optical tweezers 1, and dynamic holographic optical tweezers hot arrays 2 have provided a means. Optical trapping of threedimensional structures using dynamic holograms. Such the particle manipulating technique is called optical tweezers. Pdf optimized holographic optical traps researchgate. To do this, we trap a single particle in a multipletrap. This article describes how computergenerated holograms can dynamically reconfig ure a single optical tweezer into hundreds of independent.

Highly controllable optical tweezers using dynamic electronic holograms. Download full pdf here colloid science principles, methods and applications 4. The inset phase grating is 125 of the hologram encoding a array of traps, with white regions corresponding to local phase shifts of radians and black to 0. In addition to forming conventional optical tweezers, these methods also can sculpt the wavefront of each trap individually, allowing for mixed arrays of traps based on different modes of light, including optical vortices, axial line traps, optical bottles and optical rotators. Pdf holographic optical traps use the forces exerted by computergenerated holograms to trap. Optical tweezers based on optical fibers the fiber optical trap relies on the same principle as the optical trapping, but with the laser delivered. Here, we determine the trap stiffness of hots as they are steered in two dimensions. Pdf calibration of dynamic holographic optical tweezers. Minimizing intensity fluctuations in dynamic holographic. Stochastic dynamic trapping in robotic manipulation of.

Calibration of dynamic holographic optical tweezers for. Rather, the slm is directly imaged into the trapping plane. Osa aberration correction in holographic optical tweezers. Pdf, single atom movement with dynamic holographic optical. Holographic or diffractive optical components are widely implemented using spatial light modulators within optical tweezers to form multiple, andor modified traps. A stochastic dynamic programming framework was proposed for automated particle transport operations in 32. Rotation of single live mammalian cells using dynamic. A typical trap requires at least 1 mw of laser power, whereas the multiple traps created by holographic optical tweezers demand source powers of 500 mw to 1 w 5, 6. Highspeed holographic optical tweezers using a ferroelectric. Graduate school of bio applications and systems engineering, tokyo university of agriculture and technology, koganei, tokyo 1848588, japan. Dynamic holographic optical tweezers new york university. Holographic optical trapping and imaging the holographic optical tweezers setup used in this work has been described in detail previously. Holographic optical trapping of aerosol droplets laser quantum. Most experiments are based on spatiallycontrolled contacts between microbeads and cells.

Nanoscopy of bacterial cells immobilized by holographic. Optical tweezers systems are available commercially and they have found a number of applications,3 including measuring the compliance of bacteria,4 measuring the forces exerted by motor proteins5,6 and trapping metal nanoparticles. In this study, we present a method designed to generate dynamic holograms in holographic optical tweezers. Holographic phase contrast for dynamic multiplebeam. Dynamic holographic optical tweezers sciencedirect.

We demonstrate the advantages of a ferroelectric liquid crystal spatial light modulator for optical tweezer array applications. Schematic implementation of dynamic holographic optical tweezers using a reflective liquid crystal spatial light modulator. The approach combines our random mask encoding method with iterative highefficiency. Osa calibration of dynamic holographic optical tweezers. A telescope relays the diffracted beams to a highnumericalaperture objective which focuses them into optical traps. Application of this technique to force measurements requires calibration of trap stiffness and its position dependence. April 21, 2012 optical trapping is an increasingly. A novel control and planning approach was presented for automated and indirect.

Robertson, dynamic holography for optical interconnections. Design strategies for optimizing holographic optical tweezers. Optical tweezers, holographic optical tweezers, generalized lorenzmie theory, diffusion. We demonstrate the use of holographic optical tweezers for trapping particles in air, specifically aerosol droplets. Furthermore, holographic optical tweezers have an advantage over other methods of dynamic light array generation, such as time sharing 7, in that the modulator spatially modifies the phase of the incoming wavefronts. Investigation on force detection methods for optical trapping and. Optical tweezers technique and its applications springerlink. N2 optical trapping is an increasingly important technique for controlling and probing matter at length scales ranging from nanometers to millimeters.

Aberration correction in holographic optical tweezers. We report studies of the coalescence of pairs of picolitre aerosol droplets manipulated with holographic optical tweezers, probing the shape relaxation dynamics following coalescence by simultaneously monitoring the intensity of elastic backscattered light ebl from the trapping laser beam time resolution on the order of 100 ns while recording high frame rate camera images time resolution. Single atom movement with dynamic holographic optical tweezers. One way of creating dynamic holographic optical tweezers is to use a liquid crystal spatial light modulator, as first achieved in 1999 by yoshio hayasaki and co. Rotation of single live mammalian cells using dynamic holographic optical tweezers article pdf available in optics and lasers in engineering 92. Introduction optical tweezers are used for manipulating both single and multiple, micronsized, particles suspended in solution 1. Holographic optical tweezers hots enable the manipulation of multiple traps independently in three dimensions in real time. Minimizing intensity fluctuations in dynamic holographic optical tweezers by restricted phase change. Holographic optical tweezers elegantly extend the basic principle of optical. Such high power levels, especially in the visible wavelengths, can result in optical andor thermal. Calibration of dynamic holographic optical tweezers for force measurements on biomaterials. Pdf highspeed holographic optical tweezers using a ferroelectric.

Optical trapping is an increasingly important technique for controlling and probing matter at length scales ranging from nanometers to millimeters. Various types of particles can be trapped including transparent. Aberration correction in holographic optical tweezers kurt d. Highly controllable optical tweezers using dynamic electronic holograms volume. When implemented with a spatial light modulator, such holographic optical traps also can move objects in three dimensions. Highspeed holographic optical tweezers using a ferroelectric liquid crystal microdisplay. Mcgloin supa, school of physics and astronomy, university of st. Holographic optical tweezers hot enables highly flexible and precise control of particles, introducing holography technique to them. One way of creating dynamic holographic optical tweezers is to use a liquidcrystal spatial light modulator, as first achieved in 1999 by yoshio hayasaki and co. In contrast to dynamic holographic optical tweezers, the transfer of the phase distribution on the slm to a trapping intensity. Reid1, 5 6 1school of chemistry, university of bristol, bristol, bs8 1ts, united kingdom 7 2department of mathematics, university of leicester, leicester, le1 7rh, united kingdom. Now, 20 years after the development of hot, we present the realization of. Dynamic measurements and simulations of airborne picolitre.

Highly controllable optical tweezers using dynamic. Calibration of dynamic holographic optical tweezers for force measurements. Holographic plasmonic nano tweezers for dynamic trapping and manipulation. Assembly of 3dimensional structures using programmable. Holographic plasmonic nanotweezers for dynamic trapping. We show that by further modifying the hologram design to account for residual aberrations, the fidelity of the focused beams can be significantly improved, quantified by a spot sharpness metric. Pdf dynamic holographic optical tweezers david grier. Highspeed holographic optical tweezers using a ferroelectric liquid.